使用KaKs_calculator计算Ka/Ks

安装kakscalculator2

kakscalculator2下载地址
解压后

chmod 757 ./KaKs_Calculator2.0/src/AXTConvertor
chmod 757 ./KaKs_Calculator2.0/bin/Linux/KaKs_Calculator

添加环境变量

echo 'export PATH=/share/software/KaKs_Calculator2.0/bin/Linux:$PATH' >>~/.bashrc
echo 'export PATH=/share/software/KaKs_Calculator2.0/src:$PATH' >>~/.bashrc
source ~/.bashrc

安装序列比对软件mafft 下载地址

wget https://mafft.cbrc.jp/alignment/software/mafft-7.471-with-extensions-src.tgz
tar -zxvf mafft-7.471-with-extensions-src.tgz
cd mafft-7.471-with-extensions
cd core
vim Makefile
#手动修改prefix和bindir

我的是修改为下面的路径
PREFIX = /share/softwares/mafft
BINDIR = /share/softwares/mafft/bin

make clean
make 
make install
echo `export PATH=/share/softwares/mafft/bin:$PATH` >>~/.bashrc
source ~/.bashrc

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