CORNING Collagen I

Thin coating
We recommend using Corning Collagen I, rat tail, as a thin coating at 5μg/cm2. Please use this as a guideline for determining the optimum concentration for your application.

1. Dilute material to 50 μg/mL using 0.02 N acetic acid. Corning Collagen I, rat tail, is insoluble at neutral pH.
2. Add enough diluted material to coat dishes with 5μg/m2.
3. Incubate at room temperature for one hour.
4. Carefully aspirate remaining solution.
5. Rinse well to remove acid, using PBS or serum free medium.
6. Plates may be used immediately or may be air dried. They may be stored at 2-8℃ for up to one week under sterile conditions.