【文献速览】-Single-cell analysis of t

Human testis development in prenatal life involves complex changes in germline and somatic cell identity. To better understand, we profiled and analyzed ∼32,500 single-cell transcriptomes of testicular cells from embryonic, fetal, and infant stages. Our data show that at 6–7 weeks postfertilization, as the testicular cords are established, the Sertoli and interstitial cells originate from a common heterogeneous progenitor pool, which then resolves into fetal Sertoli cells (expressing tube-forming genes) or interstitial cells (including Leydig-lineage cells expressing steroidogenesis genes). Almost 10 weeks later, beginning at 14–16 weeks postfertilization, the male primordial germ cells exit mitosis, downregulate pluripotent transcription factors, and transition into cells that strongly resemble the state 0 spermatogonia originally defined in the infant and adult testes. Therefore, we called these fetal spermatogonia “state f0.” Overall, we reveal multiple insights into the coordinated and temporal development of the embryonic, fetal, and postnatal male germline together with the somatic niche.
产前生活中人类睾丸的发育涉及种系和体细胞身份的复杂变化。为了更好地理解，我们对来自胚胎，胎儿和婴儿阶段的睾丸细胞的约32,500个单细胞转录组进行了分析和分析。我们的数据显示，在受精后6-7周，随着睾丸索的建立，Sertoli和间质细胞起源于一个共同的异质祖细胞，然后分解为胎儿Sertoli细胞（表达成管基因）或间质细胞（包括表达类固醇生成基因的Leydig谱系细胞。大约10周后，从受精后14–16周开始，男性原始生殖细胞退出有丝分裂，下调多能转录因子，然后转变成与最初在婴儿和成人睾丸中定义的0精原细胞状态非常相似的细胞。因此，我们称这些胎儿精原细胞为“状态f0”。总体而言，我们揭示了对胚胎，胎儿和产后雄性生殖系以及体位利基的协调和时间发育的多种见解。